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All of our publications can be found on Google Scholar. Below are some recent contributions.

Subcellular Partitioning of Trace Elements Is Related to Metal Ecotoxicological Classes in Livers of Fish (Esox lucius; Coregonus clupeaformis) from the Yellowknife Area (Northwest Territories, Canada)

Aymeric Rolland, Mike Palmer, John Chételat, Marc Amyot, Maikel Rosabal
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The subcellular partitioning of trace elements (TEs) may depend on their binding preferences, although few field data are available from mining-impacted areas. Northern pike and lake whitefish were collected from different aquatic systems located in the Yellowknife mining area (Northwest Territories, Canada) to examine the subcellular partitioning of TEs in liver cells. Elements belonging to metal classes based on binding affinities were considered: A (Ce, La), borderline (As, Pb), and class B (Ag, Cd). Measurements in the metal-detoxified fractions (granule-like structures and heat-stable proteins and peptides) and in the putative metal-sensitive fractions (heat-denatured proteins, mitochondria and microsomes, and lysosomes) revealed marked differences among metal classes. In both fish species, Cd and Ag accumulated more as detoxified forms (higher than 50%, likely bound to metallothionein-like proteins) than La and Ce (not more than 20%). The two borderline TEs (As and Pb) showed an intermediate behavior between classes A and B. Similar proportions were found in the “sensitive” subcellular fractions for all TEs, where quantitative ion character-activity relationships (QICARs) indicated the covalent index and electronegativity as predictors of the TE contribution in this compartment. This study supports the use of classes of metals to predict the toxicological risk of data-poor metals in mining areas.

Diminishing Mercury Bioaccumulation in Zooplankton along an Estuarine Salinity Gradient

Annabelle Vogl, Kimberley Desjardins, Dominic E Ponton, Gesche Winkler, Marc Amyot
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Estuarine transition zones (ETZs) are biogeochemically complex, nutrient-rich environments supporting diverse and productive food webs. They may also be sites of microbial production of methylmercury (MeHg) and bioaccumulation of this neurotoxicant at the base of the food web. However, the environmental drivers controlling these mechanisms are unclear. We studied the pattern of MeHg bioaccumulation in zooplankton along the 200 km ETZ of a large North American estuary, the St. Lawrence Estuary (Québec, Canada). Our approach integrated the dynamic variation in ETZ geochemistry, focusing on MeHg speciation change, alongside ecological factors, including community composition and stable isotopic tracers of diet and habitat. MeHg bioaccumulation decreased with distance downstream along the ETZ, driven by the salinity gradient and traced by the isotopic signature of sulfur in zooplankton. MeHg speciation modeling suggested that complexation to dissolved organic matter may be used as a proxy of the trophic transfer of MeHg to zooplankton. Further, the binding of MeHg to organic matter was reduced by the presence of chloride, thus reducing the trophic transfer of MeHg. We propose a conceptual model for MeHg cycling in ETZ of large estuaries that hypothesizes that higher-level consumers in turbid upstream regions may face heightened risks of MeHg toxicity but that Hg levels diminish drastically as salinity increases.

Rare earth elements accumulation and patterns in abiotic and biotic compartments of a large river system influenced by natural and anthropogenic sources in Eastern Canada

Marie-Christine Lafrenière, Jean-François Lapierre, Dominic E Ponton, Gilbert Cabana, Gesche Winkler, Marie Lefranc, Marc Amyot
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The mobilization of rare earth elements (REEs) in aquatic ecosystems is expected to rise significantly due to intensified exploitation, erosion, and climate change. As a result, more attention has been brought to study their environmental fate. However, our ability to assess contamination risks in freshwater organisms remains limited due to scarce data on the composition and accumulation of REEs. Understanding how organisms bioaccumulate REEs requires knowledge of their environmental conditions, exposure pathways, and ecological characteristics—areas few studies have explored. In this study, we examined the fate of REEs across abiotic (water, suspended sediments, and sediments) and biotic (invertebrates and fishes) compartments in the St. Lawrence River (Canada), identifying the main drivers of their accumulation and relative composition. The results were consistent with REE biodilution along the food chain, with concentrations greater in suspended (REEs = 76.1–241.4 μg g−1) and bulk sediments (REEs = 4.2–204.2 μg g−1). Higher concentrations were found in fine-grained sediments, with a relative enrichment in middle REEs, likely due to REE adsorption onto Fe- or Mn-bearing minerals. Nonpredatory invertebrates ingesting suspended sediments, such as Ephemeroptera and Diptera larvae, exhibited higher concentrations of REEs than both filter-feeding species (i.e., mussels, polychaetes) and fish. Additionally, some amphipods displayed anomalous concentrations of gadolinium (Gd/Gd∗ = 5.7, 2.6, and 2.0), possibly originating from anthropogenic activities near Montreal Island. While fish bioaccumulated only light REEs in their liver, multiple regression models revealed how their length and the concentration of REEs in surrounding water—in dissolved form or as free ions—influenced their concentrations. Finally, benthivorous species like Moxostoma spp. and Ictalurus punctatusaccumulated more REEs compared to piscivorous Sander spp., reflecting differences in feeding behavior and trophic level. Overall, these findings provide insights into how REE concentrations and compositions varied among organisms, likely due to differences in environmental conditions and ecological characteristics.

Improving nutritional intakes and reducing metal (loid) exposures from wild fish broth among Inuit pregnant women

Tania Groleau, Mélanie Lemire, Dominic E Ponton, Marc Amyot
Wild fish broth illustration

Recipes made with local country foods are central to Inuit culture and nutrition. Recipes made with local country foods are central to Inuit culture and nutrition. Recipes are recommended for their different health benefits, such as a wild fish broth recipe that is said to help the baby's growth during pregnancy and with lactation. However, some country foods can have high concentrations of potentially toxic metal(loid)s such as mercury (Hg), arsenic (As) or cadmium (Cd), and it is unknown to what extent these are transferred to the broth. During pregnancy, there are higher risks of developing iron (Fe) and calcium (Ca) deficiencies. A simple way to optimize the nutrient content of recipes is by adding other ingredients like seaweed, bivalves (mussels and clams) or a Lucky Iron Fish® known to be rich in these nutrients. Using an experimental approach, nutrient (essential elements and fatty acids) and metal(loid) transfer to broth were studied by measuring their concentrations in ingredients and broth. Most fish, seaweeds and bivalves were important sources (>20 % of the daily intakes) of nutrients required for healthy pregnancies. Several nutrients were transferred to the broth by these ingredients, but only fish broth was an important source of nutrients. The Lucky Iron Fish was a potential source of iron when preconditioned. Total Hg concentrations were elevated in lake trout muscles and cheeks (up to 4.5 μg/g ww; >90 % in methylated form) but were not a concern in other fish species. Few metal(loid)s were transferred to the broth, except arsenic (As). Total As concentrations were high in some raw seaweeds and most broths, but the less toxic organic forms were mainly found. Overall, wild fish broths were relatively low in nutrients and toxic forms of metal(loid)s. Adding local ingredients such as seaweed and bivalves could increase nutrient intake from fish broth if consumed as a whole.

Influence of vegetative cover on snowpack mercury speciation and stocks in the greening Canadian subarctic region

Maëlys Bockhoff, Holly Marginson, Henry Ittulak, Alexandre Roy, Marc Amyot
Element behavior in food cooking and transfer

A notable greening and warming of the Arctic and Subarctic due to climate change has uncertain implications for the global cycling of mercury (Hg). Snowpacks are dynamic reservoirs for Hg susceptible to solar radiation and wind pumping, with vegetative cover potentially altering Hg photochemistry. However, the impact of northern greening on the transformation of major Hg species and on Hg stocks remain poorly understood. Temporal surface snow and snowpit sampling was conducted under tree canopies and open tundra sites at the boreal-tundra ecotone in Nunavik, Canada. Maximum (mean) concentrations of 69.1 ng/L (8.8 ng/L) total mercury (HgT) and 46.9 ng/L (5.5 ng/L) reactive mercury (HgR) were measured in forest surface snow, with maximums attributed to rapid atmospheric oxidation events. Significant post-depositional reductions were recorded in the bay, tundra, and forest (67–99% HgR) and suggested greater Hg sequestration may occur under tree canopies. Increasing methylmercury (MeHg), HgT, and dissolved organic carbon (DOC) concentrations were detected across a vegetation gradient shifting towards humic-like organic matter. Notably, springtime depth profiles presented an approximate 12-fold greater accumulation of HgT under tree canopies compared to open tundra (p < 0.01), with up to 16-times higher stocks (HgT, MeHg, DOC) at elevated vegetation density (p < 0.05). In the North, increasing vegetation cover and surface warming may favor Hg accumulation and methylation in snowpacks, facilitated by interactions with organic matter, and further enriched by the reduced wind and solar exposure experienced under forest canopies.

Methylmercury in northern pike (Esox lucius) liver and hepatic mitochondria is linked to lipid peroxidation

Kimberley Desjardins, Dominic E Ponton, François Bilodeau, Maikel Rosabal, Marc Amyot
 
Descrizione immagine

Methylmercury (MeHg) readily bioaccumulates and biomagnifies in aquatic food webs leading to elevated concentrations in fish and may thus induce toxicity. Oxidative stress is a suggested effect of MeHg bioaccumulation in fish. However, studies on how MeHg triggers oxidative stress in wild fish are scarce. The purpose of this study was to link the subcellular distribution of MeHg in the liver of northern pike from the St. Maurice River (Québec, Canada), affected by two run-of-river (RoR) dams, artificial wetlands, forest fires, and logging activity, to lipid peroxidation as an indicator of oxidative stress. We also evaluated the protective effects of the glutathione (GSH) system and selenium (Se), as they are known to alleviate MeHg toxicity. A customized subcellular partitioning protocol was used to separate the liver into metal-sensitive (mitochondria, microsome/lysosome and HDP – heat-denatured proteins) and metal-detoxified fractions (metal-rich granules and HSP – heat-stable proteins). We examined the relation among THg, MeHg, and Se concentration in livers and subcellular fractions, and the hepatic ratio of total GSH (GSHt) to oxidized glutathione (GSSG) on lipid peroxidation levels, using the concentrations of malondialdehyde (MDA), a product of lipid peroxidation. Results showed that hepatic MDA concentration was positively correlated with the combined MeHg and Se concentrations in northern pike liver (r2 = 0.88, p < 0.001) and that MDA concentrations were best predicted by MeHg associated with the mitochondria (r2 = 0.71, p < 0.001). This highlights the need for additional research on the MeHg influence on fish health and the interactions between Hg and Se in northern pike.

Effect of cooking temperature on metal concentrations and speciation in fish muscle and seal liver

Marc Amyot, Emma Husser, Kathy St-Fort, Dominic E Ponton
Descrizione immagine

Fish and marine mammals constitute a significant part of the country food diet of many Indigenous communities in Canada. These animals sometimes accumulate essential elements as well as elevated levels of toxic metals. We experimentally assessed how changes in cooking temperature (23–99 °C by boiling) modified elemental concentrations in whitefish muscle and grey seal liver (two organs commonly consumed in some northern communities). Wet and dry elemental concentrations changed linearly as a function of temperature, and two patterns were observed: methylmercury, selenium, and rare earth elements tended to remain associated with the food during cooking, whereas alkali, alkaline-earth metals, and arsenic were significantly transferred to cooking juices. Mass balances indicated that speciation of mercury was stable during cooking. Because elements generally behaved similarly as those of their periodic table group or their ecotoxicological classes (A, B, intermediate), we propose that elemental behavior during cooking is partly a function of chemical affinity, and this relationship can be used to predict the behavior of data-poor elements of emerging concern, such as technology-critical elements. Furthermore, the marked increases and decreases in elemental concentrations during cooking (e.g., −14% As and +39% Se in whitefish; −22% Cd and +55% Hg in seal liver, on a wet weight basis) should be considered when assessing risk because current exposure models usually only consider elemental concentrations in raw food.

Assessment of in vitro bioaccessibility and in vivo oral bioavailability as complementary tools to better understand the effect of cooking on methylmercury, arsenic, and …

T Charette, D Bueno Dalto, M Rosabal, JJ Matte, M AmyotToxics 9 (2), 27
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Fish consumption is the main exposure pathway of the neurotoxicant methylmercury (MeHg) in humans. The risk associated with exposure to MeHg may be modified by its interactions with selenium (Se) and arsenic (As). In vitro bioaccessibility studies have demonstrated that cooking the fish muscle decreases MeHg solubility markedly and, as a consequence, its potential absorption by the consumer. However, this phenomenon has yet to be validated by in vivo models. Our study aimed to test whether MeHg bioaccessibility can be used as a surrogate to assess the effect of cooking on MeHg in vivo availability. We fed pigs raw and cooked tuna meals and collected blood samples from catheters in the portal vein and carotid artery at: 0, 30, 60, 90, 120, 180, 240, 300, 360, 420, 480 and 540 min post-meal. In contrast to in vitro models, pig oral bioavailability of MeHg was not affected by cooking, although the MeHg kinetics of absorption was faster for the cooked meal than for the raw meal. We conclude that bioaccessibility should not be readily used as a direct surrogate for in vivo studies and that, in contrast with the in vitro results, the cooking of fish muscle did not decrease the exposure of the consumer to MeHg.

Understanding food web mercury accumulation through trophic transfer and carbon processing along a river affected by recent run-of-river dams

DE Ponton, RA Lavoie, M Leclerc, F Bilodeau, D Planas, M AmyotEnvironmental Science & Technology 55 (5), 2949-2959
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Unlike large dams which favor methylation of Hg in flooded soils over long periods, run-of-river dams are designed to flood a limited area of soils and are therefore not expected to significantly affect mercury (Hg) cycling or carbon processing. We studied the Hg and carbon cycles within food webs from several sectors along the Saint-Maurice River, Quebec, Canada, that differ in how they are influenced by two run-of-river dams and other watershed disturbances. We observed peak Hg concentrations in fish five-year postimpoundment, but these levels were reduced three years after this peak. Methylmercury concentrations in low trophic level fish and invertebrates were related to their carbon source (δ13C) rather than their trophic positions (δ15N). Biomagnification, measured by trophic magnification slopes, was driven mainly by methylmercury concentrations in low-trophic level organisms and environmental factors related to organic matter degradation and Hg-methylation. River sectors, δ13C and δ15N, predicted up to 80% of the variability in food web methylmercury concentrations. The installation of run-of-river dams and the related pondages, in association with other watershed disturbances, altered carbon processing, promoted Hg-methylation and its accumulation at the base of the food web, and led to a temporary increase in Hg levels in fish.

Microbial Diversity and Mercury Methylation Activity in Periphytic Biofilms at a Run-of-River Hydroelectric Dam and Constructed Wetlands

M Leclerc, MC Harrison, V Storck, D Planas, M Amyot, DA WalshMsphere 6 (2), e00021-21
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Monomethylmercury (MMHg) is a biomagnifiable neurotoxin of global concern with risks to human health mostly associated with fish consumption. Hydroelectric reservoirs are known to be sources of MMHg many years after their impoundment. Little is known, however, on run-of-river dams flooding smaller terrestrial areas, although their numbers are expected to increase considerably worldwide in decades to come. Production of MMHg is associated mostly with anaerobic processes, but Hg methylation has been shown to occur in periphytic biofilms located in oxic zones of the water column. Therefore, in this study, we investigated in situ production of MMHg by periphytic communities in habitats impacted by the construction of a run-of-river dam by combining transformation rate measurements with genomic approaches targeting hgcAB genes, responsible for mercury methylation. These results provide extended knowledge on mercury methylators in river ecosystems impacted by run-of-river dams in temperate habitats.

Role of organic matter and microbial communities in mercury retention and methylation in sediments near run-of-river hydroelectric dams

LM Ferriz, DE Ponton, V Storck, M Leclerc, F Bilodeau, DA Walsh, ...Science of the total environment 774, 145686
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Run-of-river power plants (RoRs) are expected to triple in number over the next decades in Canada. These structures are not anticipated to considerably promote the mobilization and transport of mercury (Hg) and its subsequent microbial transformation to methylmercury (MeHg), a neurotoxin able to biomagnify in food webs up to humans. To test whether construction of RoRs had an effect on Hg transport and transformation, we studied Hg and MeHg concentrations, organic matter contents and methylating microbial community abundance and composition in the sediments of a section of the St. Maurice River (Quebec, Canada). This river section has been affected by the construction of two RoR dams and its watershed has been disturbed by a forest fire, logging, and the construction of wetlands. Higher total Hg (THg) and MeHg concentrations were observed in the surface sediments of the flooded sites upstream of the RoRs. These peaks in THg and MeHg were correlated with organic matter proportions in the sediments (r2 = 0.87 and 0.82, respectively). In contrast, the proportion of MeHg, a proxy for methylation potential, was best explained by the carbon to nitrogen ratiosuggesting the importance of terrigenous organic matter as labile substrate for Hg methylation in this system. Metagenomic analysis of Hg-methylating communities based on the hgcA functional gene marker indicated an abundance of methanogens, sulfate reducers and fermenters, suggesting that these metabolic guilds may be primary Hg methylators in these surface sediments. We propose that RoR pondages act as traps for sediments, organic matter and Hg, and that this retention can be amplified by other disturbances of the watershed such as forest fire and logging. RoR flooded sites can be conducive to Hg methylation in sediments and may act as gateways for bioaccumulation and biomagnification of MeHg along food webs, particularly in disturbed watersheds.

Photooxidation of arsenic in pristine and mine-impacted Canadian subarctic freshwater systems

M Amyot, D Bélanger, DF Simon, J Chételat, M Palmer, P AriyaJournal of Hazardous Materials Advances 2, 100006
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The redox transformations of arsenic (As) in lakes are thought to be mainly controlled by algal/microbial processes. We tested if photochemical oxidation could also be a significant redox process in northern freshwater systems. We exposed filtered natural water samples from four northern mine-impacted lakes (Northwest Territories, Canada) and three unimpacted ponds (Nunavik, Canada) to simulated solar radiation, and followed As(III) oxidation. During short-term incubation experiments (less than 8 h), all samples amended with As(III) displayed a rapid photooxidation (ca. 0.06-0.27 h−1) whereas no significant oxidation was observed in the dark. This photooxidation process was controlled by UV radiation, was influenced by short-lived oxidants and was partly driven by the concentrations of organic matter. Natural As complexes in unamended water samples were more rapidly photooxidized than in samples with As(III) amendments. Addition of goethite or simulation of snowmelt did not alter photooxidation rates significantly. Overall, these results imply that photooxidation of As should be considered as a potentially significant mechanism controlling inorganic As speciation in aquatic systems. This is particularly true for northern systems affected by mining activities and by changes of inputs of organic matter related to climate change.